Both acidic placental form (GST-Pi) and basic liver form(GST-L) of human glutathione S-transferase were studied in the developing, normal and abnormal livers uing immunohistochemical peroxidase and antiperoxidase method. Time of appearance,
temporal
change of the activity and distribution pattern were particularly examined using 27 cases of human fetuses of ranging in age from 2 to 32 gestation weeks. 5 cases of normal infant as well as 12 cases of cirrhosis or carcinomas of the liver.
@ES The results were as follows:
@EN 1. Expression of the GST-Pi in the liver was weak in fetuses between 12 to 16 weeks but became conspicuous between 17 to 21 weeks and then it decreased to show weak posititivy between 24 to 32 weeks. The staining reaction was mild in
childhood
and
adult livers. Meanwhile, GST-L was stained weakly in fetuses between 12 to 14 weeks but became conspicuous from 14th week and of staining reaction was mild to moderate noted thereafter, in childhood and adult.
2. Expression of the GST-Pi in the bile ducts was weak in fetus between 12 to 16 weeks and became conspicuous in 17 to 24 weeks and stained strongly between 26 to 32 weeks and the strong of reaction was noted in childhood and adult intrahepatic
bild
ducts. GST-L was not demonstrated through the fetal period from 12 to 32 weeks but weakly positive in the large bile duct epithelium of childhood and adult.
3. Expression of the GST-Pi was the same degree of positivity as the normal in the normal in the hepatocytes and bile ducts adjacent to the hepatocellular carcinoma. GST-Pi showed moderately in the hepatocytes of regenerating nodule and adjacent
proliferating bile ducts. It was weak to strong in trabe cular and acinar hepatocellular hepatoblastoma.
Meanwhile, GST-L was of positive as the normal hepatocytes and bild ducts adjacent to the hepatocellular carcinoma of the liver. GST-L is weakly positive in regenerating nodule and weak to moderate in trabecular hepatocellular caricinoma, and
negligible
in acinar type.
In conclusion, It was suggested that maturation and distribution pattern of GST-Pi and GST-L are significantly difference between normal and neoplastic condition, and a possibility of dianostic application of isozymes of glutathione S-transferase
in the
differential diagnosis of neoplasms of hepatobiliary system could be raised.
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